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Day One | Day Two | Day Three | Day Four | Day Five
| DAY ONE: 4 April 2011 (Monday) |
|
Time
|
Activities
|
Trainer
|
|
09:00
|
Introduction to ChIA-PET
|
|
|
10:00
|
Workshop
|
|
|
10:15
|
COFFEE BREAK
|
|
10:30
|
Demo
|
|
|
10:40
|
Participants Perform Blunt Ends
|
|
|
10:55
|
Demo Linker Ligation
|
|
|
11.30
|
Participants Set Linker Ligation, Q&A
|
|
|
12.00
|
LUNCH
|
|
13:00
|
Analysis Workshop
|
|
|
15:30
|
COFFEE BREAK
|
|
15:50
|
Analysis Workshop (continued)
|
|
|
17:00
|
End of day one
|
|
|
19:00
|
WELCOME DINNER
|
Back to top
| DAY TWO: 5 April 2011 (Tuesday) |
|
Time
|
Activities
|
Trainer
|
|
09:00
|
Demo
|
|
|
09:30
|
Participants set PNK reaction
|
|
|
10:15
|
COFFEE BREAK
|
|
10:30
|
Participants Elute Chromation DNA
|
|
|
10:50
|
Participants Wash and Neutralize
|
|
|
12:05
|
Circularization
|
|
|
12:15
|
LUNCH
|
|
13:45
|
Analysis Workshop
|
|
|
15:30
|
COFFEE BREAK
|
|
15:50
|
Analysis Workshop (continued)
|
|
17:30
|
End of day two
|
|
Back to top
| DAY THREE: 6 April 2011 (Wednesday) |
|
Time
|
Activities
|
Trainer
|
|
09:00
|
Analysis Workshop
|
|
|
10:15
|
COFFEE BREAK
|
|
10:30
|
Analysis Workshop (continued)
|
|
|
12:00
|
LUNCH
|
|
13:30
|
Proteinase K for Degradation of Protein
|
|
|
14:00
|
Analysis Workshop
|
|
|
15:30
|
COFFEE BREAK
|
|
15:50
|
Analysis Workshop (continued)
|
|
|
17:30
|
End of day three
|
|
Back to top
| DAY FOUR: 7 April 2011 (THURSDAY) |
|
Time
|
Activities
|
Trainer
|
|
09:00
|
Demo
|
|
|
10:30
|
COFFEE BREAK
|
|
10:45
|
Thawing
|
|
|
11:00
|
Spinning of Sample
|
|
|
11:30
|
Wash, transfer and Mmel digestion
|
|
|
12:15
|
LUNCH
|
|
13:15
|
Analysis Workshop
|
|
|
14:15
|
Demo
|
|
|
14:30
|
Binding of DNA to Dynabeads
|
|
|
15:15
|
COFFEE BREAK
|
|
15:30
|
Addition of Adapters
|
|
|
17:30
|
End of day four
|
|
Back to top
| DAY FIVE: 8 April 2011 (FRIDAY) |
|
Time
|
Activities
|
Trainer
|
|
09:00
|
Nick Translation, PCR
|
|
|
10:45
|
COFFEE BREAK
|
|
11:00
|
Nick Translation, PCR (continued)
|
|
|
14:00
|
LUNCH
|
|
15:00
|
Demo for Scale-up & gel cut using old samples (only 4 can view the cutting of 2 lanes at each time, ie. 14 lanes, 4 gels)
|
|
|
16:30
|
COFFEE BREAK
|
|
16:45
|
Demo gel-crush and Aligent DNA 1000 (4 can view at 1 time, split 2 groups as we have 2 instruments) – 3 sessions of preparing the chip but just run once on the machine
|
|
|
18:15
|
End of day five
|
|
|
19:00
|
FAREWELL DINNER
|
