The major research focus of my group is understanding the relationship between genotype and phenotype. This includes research in personalized medicine, understanding disease mutations, and working with next-generation sequencing technologies and protein sequence.

Personalized Genomics
I have analyzed the personal genome of Craig Venter in great detail. I have shown that genome sequencing can help improve individual drug prescriptions by studying Dr. Craig Venter’s and Dr. Jim Watson’s genomes. I have also recommended guidelines to direct-to-consumer genetic companies such as 23andMe and Navigenics for improving disease risk predictions.

Understanding Disease Mutations
A large proportion of Mendelian disease can be attributed to mutations in coding genes. I created the SIFT algorithm (http://sift-dna.org) to predict the effect an amino acid substitution has on protein function. The SIFT algorithm is often applied to nonsynonymous variants.

Technology
As a Senior Bioinformaticist at Illumina, I designed the content for the microarrays used in genome-wide association studies. Later, I assessed next-generation sequencing technologies to observe what can be expected of the new technologies.

2002	University of Washington, PhD in Bioengineering
1997	Caltech, B.S. in Biology with Honors

2010-	Group Leader, Genome Institute of Singapore
2009-2010	Assistant Professor, J. Craig Venter Institute
2006-2009	Senior Scientist, J. Craig Venter Institute
2005-2006	Senior Scientist, Illumina
2003-2005	Bioinformatics Scientist II, Illumina
2002-2003	Bioinformatics Scientist I, Illumina
2002	Computational Research Associate, Fred Hutchinson Cancer Research Center

2009-2010	SAIC Contract: High throughput next generation sequencing of T cell receptors
2008-2010	NHGRI R01 grant: Continued Development and Maintenance of Software
2009	Most viewed BioMed Central article (in top 10 for the year)
2005	Illumina Values Award
2002	Scholarship winner, Keystone Symposia: Human Genetics and Genomics
1999-2002	Department of Energy Computational Science Graduate Fellowship
1998-1999	National Science Foundation Fellowship
1996-1998	Tau Beta Pi Engineering Honor Society

5 years	n-Small Cell Lung Cancer: Targeting Cancer Stem Cell and Drug Resistance by TCR (0)
3 years	POLARIS by BMRC (15M)
1 year	Appistry by BMRC (2M)

1. Ng PC, Murray SS, Levy S. Venter JC (2009) "An agenda for personalized medicine" Nature 461:724-726. To download pdf, click on link below: Supplemental Data File     
   


2. Kumar P, Henikoff S, Ng PC. (2009) Predicting the effects of coding nonsynonymous variants on protein function using the SIFT algorithm Nature Protocols 4:1073-81. To download pdf, click on link below: Supplemental Data File     
   


3. Harismendy O*, Ng PC*, Strausberg RL, Wang X, Stockwell TB, Beeson KY, Schork NJ, Murray SS, Topol EJ, Levy S, Frazer KA "Evaluation of next generation sequencing platforms for population targeted sequencing studies." Genome Biol 2009 ; 10(3) : R32. *These authors contributed equally. Epub 2009 Mar 27 Abstract      Supplemental Link     
   


4. Ng PC, Zhao Q, Levy S, Strausberg RL, Venter JC "Individual genomes instead of race for personalized medicine." Clin Pharmacol Ther 2008 Sep ; 84(3) : 306-9. To download pdf, click on link below: Abstract      Supplemental Data File     
   


5. Ng PC, Levy S, Huang J, Stockwell TB, Walenz BP, Li K, Axelrod N, Busam DA, Strausberg RL, Venter JC "Genetic variation in an individual human exome." PLoS Genet 2008 ; 4(8) : e1000160 Abstract      Supplemental Link     
   


6. Levy S, Sutton G, Ng PC, Feu k L, Halpern AL, Walenz BP, Axelrod N, Huang J, Kirkness EF, Denisov G, Lin Y, MacDonald JR, Pang AW, Shago M, Stockwell TB, Tsiamouri A, Bafna V, Bansal V, Kravitz SA, Busam DA, Beeson KY, McIntosh TC, Remington KA, Abril JF, Gill J, Borman J, Rogers YH, Frazier ME, Scherer SW, Strausberg RL, Venter JC "The diploid genome sequence of an individual human." PLoS Biol 2007 Sep 4 ; 5(10) : e254 Abstract      Supplemental Link     
   


7. Ng PC, Henikoff S "Predicting the effects of amino acid substitutions on protein function." Annu Rev Genomics Hum Genet 2006 ; 7 : 61-80. To download pdf, click on link below: Abstract      Supplemental Data File     
   


8. Ng PC, Henikoff S "SIFT: Predicting amino acid changes that affect protein function." Nucleic Acids Res 2003 Jul 1 ; 31(13) : 3812-4 Abstract      Supplemental Link     
   


9. Ng PC, Henikoff S "Accounting for human polymorphisms predicted to affect protein function." Genome Res 2002 Mar ; 12(3) : 436-46 Abstract      Supplemental Link     
   


10. Ng PC, Henikoff S "Predicting deleterious amino acid substitutions." Genome Res 2001 May ; 11(5) : 863-74 Abstract      Supplemental Link