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RESEARCH FOCUS
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In general, transcriptional activation results from the tethering of sequence specific DNA binding transcription factors to their cognate sites. This leads to the assembly of transcription initiation complex at promoters and subsequent transcription elongation. The mammalian genome encodes for over 2000 transcription regulators, and many of them play critical roles in orchestration of gene expression which is fundamental to cellular phenotypes and identity of the organism. Our long term goal is to dissect the functional role of transcription regulators using two complementary technologies (1. chromatin immunoprecipitation to study the location of transcription factors in living cells, and 2. RNAi depletion to examine the response of cells in the absence of the factors). This experimental strategy will allow us to probe the functional importance of the transcription factors of interest. We have setup a system for large scale RNAi to screen for transcription factors (or other proteins) involved in the regulation of cell-type specific promoter. Complemented with ChIP, we are able to dissect the functional importance of binding of transcription factors to key regulatory elements.
Our focus is on stem cell biology. We are addressing two questions:
1) What makes a stem cell a stem cell?
2) How to make a non-stem cell a stem cell?
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EDUCATION
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| 1996-1999 | PhD. University of Edinburgh Advisor: Adrian Bird
| | 1992-1996 | B.Sc (Honours 1st class) National University of Singapore Advisor: Eng Chong Pua and Ben Li
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PROFESSIONAL APPOINTMENTS
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| 2007- | Senior Group Leader, Genome Institute of Singapore |
| 2008- | Adjunct Associate Professor (School of Biological Sciences, Nanyang Technological University) |
| 2009- | Adjunct Associate Professor (Department of Biochemistry, National University of Singapore) |
| 2009- | Adjunct Associate Professor (Department of Biological Sciences, National University of Singapore) |
| 2003-2007 | Group Leader, Genome Institute of Singapore |
| 2003-2009 | Assistant Professor (Department of Biological Sciences, National University of Singapore) |
| 2000-2003 | Postdoctoral fellow, Harvard Medical School Advisor: Kevin Struhl
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HONORS AND AWARDS
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| 2010 | Singapore Youth Award (Medal of commendation) for Science and Technology |
| 2010 | Chen New Investigator Award (Chens’ Foundation and Human Genome Organisation) |
| 2009 | The Young Outstanding Persons Award of Singapore (Scientific and/or technological development) |
| 2008 | Outstanding Scientist Award (Faculty of Science, National University of Singapore) |
| 2007 | National Science Award |
| 2005 | Singapore Youth Award (Individual) for Science and Technology |
| 2004 | Singapore Young Scientist Award |
| 2003 | BMRC Young Investigator Award |
| 2000 | Damon Runyon-Walter Winchell Postdoctoral fellowship |
| 1996 | Darwin Trust of Edinburgh Scholarship |
| 1996 | Lijen Industrial Development Medal (National University of Singapore) |
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EXTERNAL GRANT
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2006 to 2009 |
Human Stem Cell Epigenome Project by
SSCC, BMRC (-)
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SELECTED PUBLICATIONS
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1. Heng JC, Feng B, Han J, Jiang J, Kraus P, Ng JH, Orlov YL, Huss M, Yang L, Lufkin T, Lim B, Ng HH "The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to pluripotent cells." Cell Stem Cell 2010 Feb 5 ; 6(2) : 167-74 Epub 2010 Jan 21
Abstract
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2. Feng Bo, Ng Jia-Hui, Heng Jian-Chien Dominic, Ng Huck-Hui "Molecules that Promote or Enhance Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells." Cell Stem Cell 2009 Apr 3 ; 4(4) : 301-12
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3. Feng Bo, Jiang Jianming, Kraus Petra, Ng Jia-Hui, Heng Jian-Chien Dominic, Chan Yun-Shen, Yaw Lai-Ping, Zhang Weiwei, Loh Yuin-Han, Han Jianyong, Vega Vinsensius B, Cacheux-Rataboul Valere, Lim Bing, Lufkin Thomas, Ng Huck-Hui "Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb." Nat. Cell Biol. 2009 Feb ; 11(2) : 197-203
Abstract
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4. Yuan Ping, Han Jianyong, Guo Guoji, Orlov Yuriy L, Huss Mikael, Loh Yuin-Han, Yaw Lai-Ping, Robson Paul, Lim Bing, Ng Huck-Hui "Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells." Genes Dev. 2009 Nov 1 ; 23(21) : 2507-20
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5. Jiang Jianming, Chan Yun-Shen, Loh Yuin-Han, Cai Jun, Tong Guo-Qing, Lim Ching-Aeng, Robson Paul, Zhong Sheng, Ng Huck-Hui "A core Klf circuitry regulates self-renewal of embryonic stem cells." Nat. Cell Biol. 2008 Mar ; 10(3) : 353-60
Abstract
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6. Chen Xi, Xu Han, Yuan Ping, Fang Fang, Huss Mikael, Vega Vinsensius B, Wong Eleanor, Orlov Yuriy L, Zhang Weiwei, Jiang Jianming, Loh Yuin-Han, Yeo Hock Chuan, Yeo Zhen Xuan, Narang Vipin, Govindarajan Kunde Ramamoorthy, Leong Bernard, Shahab Atif, Ruan Yijun, Bourque Guillaume, Sung Wing-Kin, Clarke Neil D, Wei Chia-Lin, Ng Huck-Hui "Integration of external signaling pathways with the core transcriptional network in embryonic stem cells." Cell 2008 Jun 13 ; 133(6) : 1106-17
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7. Zhao Xiao Dong, Han Xu, Chew Joon Lin, Liu Jun, Chiu Kuo Ping, Choo Andre, Orlov Yuriy L, Sung Wing-Kin, Shahab Atif, Kuznetsov Vladimir A, Bourque Guillaume, Oh Steve, Ruan Yijun, Ng Huck-Hui, Wei Chia-Lin "Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells." 2007 Sep 13 ; 1(3) : 286-98
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8. Loh Yuin-Han, Zhang Weiwei, Chen Xi, George Joshy, Ng Huck-Hui "Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells." Genes Dev. 2007 Oct 15 ; 21(20) : 2545-57
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9. Lim Ching-Aeng, Yao Fei, Wong Joyce Jing-Yi, George Joshy, Xu Han, Chiu Kuo Ping, Sung Wing-Kin, Lipovich Leonard, Vega Vinsensius B, Chen Joanne, Shahab Atif, Zhao Xiao Dong, Hibberd Martin, Wei Chia-Lin, Lim Bing, Ng Huck-Hui, Ruan Yijun, Chin Keh-Chuang "Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation." Mol. Cell 2007 Aug 17 ; 27(4) : 622-35
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10. Loh Yuin-Han, Wu Qiang, Chew Joon-Lin, Vega Vinsensius B, Zhang Weiwei, Chen Xi, Bourque Guillaume, George Joshy, Leong Bernard, Liu Jun, Wong Kee-Yew, Sung Ken W, Lee Charlie W H, Zhao Xiao-Dong, Chiu Kuo-Ping, Lipovich Leonard, Kuznetsov Vladimir A, Robson Paul, Stanton Lawrence W, Wei Chia-Lin, Ruan Yijun, Lim Bing, Ng Huck-Hui "The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells." Nat. Genet. 2006 Apr ; 38(4) : 431-40
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COMPLETE PUBLICATION LIST
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